The thyronine’s act on the body to increase the basal metabolic rate, affect protein synthesis and increase the body’s sensitivity to catecholamines (such as adrenaline) by permissiveness. The thyroid hormones are essential to proper development and differentiation of all cells of the human body. T4 and T3 are transported through the peripheral blood stream largely bound to serum proteins. The major transport protein is Thyroxin Binding Globulin (TBG) which normally accounts for 80% of the bound hormone. The other thyroid hormone binding proteins are Thyroxin Binding Prealbumin and Albumin. Only about 0.3% of the total serum T3 and only about 0.1% of the total serum T4 are unbound and free to diffuse into tissue to exert their biological effects. When the level of TBG increases, the level of total T4 and T3 will increase to maintain the same level of unbound or free T4 and T3 in the bloodstream of an euthyroid individual.
Simply determining the total T4 or total T3 concentration fails to take into account the variations in TBG levels that affect the unbound hormone (free T4 and free T3) concentration. TBG levels can vary for reasons incidental to the patient’s thyroid status such as the presence of certain drugs, steroid hormones, pregnancy, and various non-thyroidal diseases.
|Required Information About FT4|
|Conjugate Solution||T4-HRP||Ready To Use|
|Chromogen- Substrate||Tetramethylbenzidine (TMB) and hydrogen peroxide||Ready To Use|
|Incubation time||45 + 15 min||Room temperature|
|Standards||ng/dl 0.0 ,0.5 ,1.0 ,2.5 ,5.0 ,10||Ready To Use|
|Required Samplers||μl 25 ,50 ,100|
|Control serum 1||Normal control||Ready To Use|
|Stop Solution||Hydrochloric acid(1M)||Ready To Use|
|Wash Solution||PBS - Tween||(1vial, 30 ml concentrated,20x)|
|Plate||coated with monoclonal anti -T4||Wells 48 - 96|