Thyroid hormone uptake (Tuptake or T3 uptake) is a measure of the unbound thyroxine binding globulins in the blood, that is, the TBG that is unsaturated with thyroid hormone
Unsaturated TBG increases with decreased levels of thyroid hormones. It is not directly related to triiodothyronine, despite the name T3 uptake
| Required Information About T.U.P | ||
|---|---|---|
| Assay Type | Competitive | |
| Conjugate Solution | T4-HRP | Ready To Use |
| Chromogen- Substrate | Tetramethylbenzidine (TMB) and hydrogen peroxide | Ready To Use |
| Incubation time | 45 + 15 min | Room temperature |
| Standards | 44% , 34% , 24% , 14% | Ready To Use |
| Required Samplers | μl 25 ,50 ,100 | |
| Control serum 1 | Normal control | Ready To Use |
| Stop Solution | Hydrochloric acid(1M) | Ready To Use |
| Wash Solution | PBS - Tween | (1vial, 30 ml concentrated,20x) |
| Plate | coated with monoclonal anti -T4 | Wells 48 - 96 |