T3

Product Features

  • No need for shaker
  • Calibrated to WHO international Standard
  • Single - step test method
  • 48 , 96 , 192 test packages
  • Ready to use solutions

Product Catalog

The triiodothyronine (T3), is a tyrosine-based hormones produced by the thyroid gland. An important component in the synthesis is iodine. Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only the free fraction of T3 is biologically active, a very small fraction of the circulating hormone is unbound – T3 0.3%. The thyronines act on the body to increase the basal metabolic rate, affect protein synthesis and increase the body’s sensitivity to catecholamines (such as adrenaline) by permissiveness. The thyroid hormones are essential to proper development and differentiation of all cells of the human body. These hormones also regulate protein, fat, and carbohydrate metabolism, affecting how human cells use energetic compounds. Numerous physiological and pathological stimuli influence thyroid hormone synthesis.Both excess and deficiency of thyroxine can cause disorders. Thyrotoxicosis or hyperthyroidism is the clinical syndrome caused by an excess of circulating free thyroxine, free triiodothyronine, or both. It is a common disorder that affects approximately 2% of

women and 0.2% of men. Hyperthyroidism is the case where there is a deficiency of thyroxine.

Release of T3 from the thyroid gland into the blood is ~5-8 micrograms/day. In addition, approximately 22

mg/day of T3 is produced by nonthyroidal 5- monodeionination of T4. T3 has a much faster turnover than T4 (T ~1 day, compared to ~6 days for T4) and has greater biological potency than T4. Several drugs are known to affect the binding of Triiodothyronine to the thyroid hormone carrier proteins or its metabolism to T3 and complicate the interpretation of T3 results. Circulating autoantibodies to T3 and hormone-binding inhibitors may interfere. Measurement of total serum T3 concentrations is a standard

Required Information About T3
Assay Type Competitive
Conjugate Solution T3-HRP Ready To Use
Chromogen- Substrate Tetramethylbenzidine (TMB) and hydrogen peroxide Ready To Use
Incubation time 45 + 15 min Room temperature
Standards ng/ml 0.0, 0.5 ,1.0 ,2.5 ,5.0 ,10 Ready To Use
Required Samplers μl 50,100
Control serum 1 Normal control Ready To Use
Stop Solution Hydrochloric acid(1M) Ready To Use
Wash Solution PBS - Tween (1vial, 30 ml concentrated,20x)
Plate monoclonal anti –T3 Wells 48 - 96
Sensitivity ng/ml 0.2

Product Features

  • No need for shaker
  • Calibrated to WHO international Standard
  • Single - step test method
  • 48 , 96 , 192 test packages
  • Ready to use solutions

Product Catalog