The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5′- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a very small fraction of the circulating hormone is free (unbound) – T4 0.03%. The thyronines act on the body to increase the basal metabolic rate, affect protein synthesis and increase the body’s sensitivity to catecholamines (such as adrenaline) by permissiveness. The thyroid hormones are essential to proper development and differentiation of all cells of the human body. These hormones also regulate protein, fat, and carbohydrate metabolism, affecting how human cells use energetic compounds. Numerous physiological and pathological stimuli influence thyroid hormone synthesis. Thyrotoxicosis or hyperthyroidism is the clinical syndrome caused by an excess of circulating free thyroxine, free triiodothyronine, or both. Both T3 and T4 are used to treat thyroid hormone deficiency (hypothyroidism).
| Required Information About T4 | ||
|---|---|---|
| Assay Type | Competitive | >|
| Conjugate Solution | T4-HRP | Ready To Use |
| Chromogen- Substrate | Tetramethylbenzidine (TMB) and hydrogen peroxide | Ready To Use |
| Incubation time | 45 + 15 min | Room temperature |
| Standards | µg/dl 0.0 ,2.5 ,5.0 ,10 ,15 ,30 | Ready To Use |
| Required Samplers | μll 25 ,50 ,100 | |
| Control serum 1 | Normal control | Ready To Use |
| Stop Solution | Hydrochloric acid(1M) | Ready To Use |
| Wash Solution | PBS - Tween | (1vial, 30 ml concentrated,20x) |
| Plate | coated with monoclonal anti -T4 | Wells 48 - 96 -192 |
| Sensitivity | µg/dl 0.2 | |